Analytical service

The Analytical service offer includes analytical monitoring of biotechnology projects. In addition, we provide advice and support in developing analytical methods and conducting analytical campaigns.

Manager: Emmanuel Desmartin, emmanuel.desmartin@inrae.fr

Analytical activity

In support of the projects carried out at TWB, the analytical service provides advice, develops analytical methods, carries out analysis campaigns, trains and provides equipment to TWB facilities users.

The service’s activity is mainly focused on the quantitative and qualitative monitoring of transformation and bioconversion processes (enzymes, micro-organisms), as well as on the analytical monitoring of screening (strains, culture conditions).

Validated methods available for the assay of substrates, products and metabolites: sugars, organic acids, amino acids, salts, etc.
Development and validation of fit-to-purpose methods, or transfer of methods
Sample preparation (lyophilisation, liquid/liquid extraction, SPE, etc.) prior to analysis
Non-targeted analysis by high-resolution mass spectrometry
Qualitative analysis of polymers in solution (molar mass, size, distribution)
Purification of molecules of interest (recombinant proteins and enzymes in particular) on a semi-preparative scale
Fermentation assessment by elemental analysis of culture media and broths
Provision of analytical equipment

Analytical service equipment

Liquid chromatography (HPLC, UHPLC, SEC) coupled with various detections: UV, DAD, Refractometry (RI), Fluorescence ; Corona (CAD) ; MALS ; Mass spectrometry (MS, MS/MS and Orbitrap)
Ion chromatography (HPIC), with electrochemical (amperometry), conductimetry and UV detection
Ionscan* platform: analysis of fermentation musts by ion chromatography
Gas chromatography (GC), with FID or MS detection
Elemental analysis (COT/NT and CHONS)

Types of collaboration

The analytical service is open to collaboration with academic and industrial partners. These collaborations involve the provision of services or collaborative R&D projects. They also focus on service and assistance for research projects.